***** seminarMLから情報転載 *****

下記のように臨床研セミナーを開催します。Gilbert教
授は、DNA複製の発生過程での制御、エピジェネティッ
ク制御の研究で大変著名です。今回分子生物学会に招
聘され、来日されますのでこの機会に最新の研究成果
についてお話いただきます。事前連絡なしにご自由に
ご参加ください。なお臨床研は、上北沢に引っ越しま
した。最寄りは京王線上北沢駅です。
http://www.rinshoken.or.jp/access/index.html
をご参照ください。お間違えのないように。


日時: 平成21年 12月 7日 (月曜日) 午前11:00 ~ 12:00

会場: (財)東京都医学研究機構 東京都臨床医学総合
研究所 2階講堂

演題:REPLICATION TIMING AS AN EPIGENETIC FINGERPRINT OF STEM CELL
IDENTITY

(幹細胞のidentityを規定するエピゲノムマークとして
の複製タイミング)

演者 : David M. Gilbert教授 (Departments of Biological Science&s_comma;
Florida State University&s_comma; Tallahassee&s_comma; Florida 32306 USA)



《要旨》

We have constructed high-resolution genome-wide replication timing
profiles of mouse and human embryonic stem cells (ESCs)&s_comma; their
differentiated counterparts&s_comma; fetal and adult cell types. Replication
profiles are highly cell type specific; widespread developmentally
programmed changes in replication timing occur at the level of 4-800
kb chromosome segments. Cell lines modeling stages of the post-
implantation epiblast indicate that early development involves
extensive lineage-independent early-to-late (EtoL) replication timing
switches that are completed prior to germ layer specification and down-
regulation of key pluripotency transcription factors. These changes
remain stable in all subsequent cell lineages and involve a class of
irreversibly silenced genes. Lineage-specific&s_comma; mostly late-to-early
(LtoE) replication switches follow&s_comma; creating cell-type specific
replication profiles. Importantly&s_comma; partially reprogrammed murine iPSCs
reproducibly fail to restore mESC-specific replication timing and
transcription programs within regions of lineage-independent
replication timing changes. Interestingly&s_comma; human ESC replication
profiles were considerably more aligned with those from mouse epiblast-
derived stem cells (mEpiSCs) than with mouse ESCs. Together&s_comma; our
results demonstrate the power of replication profiling to identify
important epigenetic distinctions between closely related stem cell
populations and identify replication-timing switches occurring in the
epiblast that embody an epigenetic commitment to differentiation prior
to germ layer specification.



〔世話人:ゲノム動態プロジェクト 正井 久雄〕


Hisao Masai
Genome Dynamics Project&s_comma;
Tokyo Metropolitan Institute of Medical Science&s_comma;
2-1-6 Kamikitazawa&s_comma; Setagaya-ku&s_comma; Tokyo 156-8506&s_comma; JAPAN
Tel: 81-3-5316-3231 Fax: 81-3-5316-3145; E-mail: masai-hs@igakuken.or.jp
http://www.rinshoken.or.jp/CB/index-jp.htm

正井 久雄
東京都臨床医学総合研究所 ゲノム動態プロジェクト
郵便番号 156-8506
所在地  東京都世田谷区上北沢二丁目1番6号
正井直通電話 03-5316-3231
研究室電話 03-5316-3117
研究室FAX 03-5316-3145
E-mail masai-hs@igakuken.or.jp



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