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神経生化学セミナーのご案内/Neurochemistry Seminar Announcement

Title: "Divergent regulation by RNA editing: microRNA function&s_comma; energy metabolism&s_comma; and beyond"
Lecturer: Yukio Kawahara&s_comma; MD&s_comma; PhD
Affiliation: The Wistar Institute&s_comma; Philadelphia&s_comma; PA&s_comma; USA

Time: August 1&s_comma; 2007 (Wed) 12:00~13:00
Place: Room 202 (Seminar Room 2)&s_comma; 2nd floor&s_comma; New Medical Research Building
Host: Haruhiko Bito&s_comma; Department of Neurochemistry (03-5841-3560)
Supported by Neuroscience Lecture Series&s_comma; Center for Integrated Brain Medical
Science&s_comma; a 21st Century COE Program from MEXT.

RNA editing that converts adenosine residues to inosine in double-stranded regions is a genetic mechanism for generating RNA and protein diversity. In addition to several cases of editing in protein-coding RNAs&s_comma; such as glutamate receptor subunits and serotonin 5-HT2C receptor&s_comma; recent evidence indicates that
a large number of RNA editing sites are located in untranslated RNA sequences&s_comma;and non-coding RNA genes&s_comma; including primary transcripts of certain microRNAs.
While we recently revealed the importance of RNA editing in microRNA-mediated gene silencing&s_comma; the physiological significance of RNA editing remains unknown in most cases. 5-HT2CR has 5 editing sites in it’s coding regions&s_comma; leading to expression of up to 24 different isoforms of this receptor&s_comma; with alteration of functions such as G-protein coupling&s_comma; agonist affinity&s_comma; and receptor trafficking in vitro. Therefore&s_comma; alteration of the RNA editing pattern has been associated
with major psychiatric disorders. 10 years after 5-HT2CR editing was identified&s_comma; in order to better understand the in vivo significance of 5-HT2CR editing&s_comma; we created two mutant mouse lines harboring a knocked-in allele that results in sole expression of the fully edited or unedited isoform. These mutant mice reveal various unexpected phenotypes&s_comma; including the alteration of energy metabolism&s_comma; which I would like to introduce in this seminar to discuss in depth.

References;
1) Kawahara et al&s_comma; Science&s_comma; 315: 1136-1140&s_comma; 2007.
2) Kawahara et al&s_comma; EMBO Rep.&s_comma; doi: 10.1038/sj.embor.7401011&s_comma; 2007.
3) Kawahara et al&s_comma; Nature&s_comma; 427: 801&s_comma; 2004.

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尾藤晴彦
東京大学大学院医学系研究科
脳神経医学専攻 神経生化学分野
〒113-0033 東京都文京区本郷7-3-1
e-mail: hbito@m.u-tokyo.ac.jp
Tel: 03-5841-3559 Fax: 03-3814-8154





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