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 米国Wistar Instituteの西倉和子教授の研究室にて、先端的なRNA編集に関し
講師 米国Wistar Institute 河原行郎博士
日時 2007年7月27日 14:00 - 15:00
場所 理研脳科学センター(埼玉県和光市)
   中央棟 4階セミナー室
参加無料、事前登録不要 (セミナーは英語で行われます)

Divergent regulation by RNA editing:
microRNA function&s_comma; locomotion&s_comma; and beyond.

Yukio Kawahara&s_comma; MD&s_comma; PhD
The Wistar Institute&s_comma; Philadelphia&s_comma; PA&s_comma; USA

Date: July 27/2007
Time: 14:00 - 15:00
Place: BSI Central Building 4th floor seminar room

RNA editing that converts adenosine residues to inosine in double-
stranded regions is a genetic mechanism for generating RNA and protein
diversity. In addition to several cases of editing in protein-coding
RNAs&s_comma; such as glutamate receptor subunits and serotonin 5-HT2C receptor&s_comma;
recent evidence indicates that a large number of RNA editing sites are
located in untranslated RNA sequences&s_comma; and non-coding RNA genes&s_comma;
including primary transcripts of certain microRNAs. While we recently
revealed the importance of RNA editing in microRNA-mediated gene
silencing&s_comma; the physiological significance of RNA editing remains unknown
in most cases. 5-HT2CR has 5 editing sites in it’s coding regions&s_comma;
leading to expression of up to 24 different isoforms of this receptor&s_comma;
with alteration of functions such as G-protein coupling&s_comma; agonist
affinity&s_comma; and receptor trafficking in vitro. Therefore&s_comma; alteration of
the RNA editing pattern has been associated with major psychiatric
disorders. Ten years after 5-HT2CR editing was identified&s_comma; in order to
better understand the in vivo significance of 5-HT2CR editing&s_comma; we
created two mutant mouse lines harboring a knocked-in allele that
results in sole expression of the fully edited or unedited isoform.
These mutant mice reveal various unexpected phenotypes&s_comma; including the
alteration of locomotion&s_comma; which I would like to introduce in this
seminar to discuss in depth.

1) Kawahara et al&s_comma; Science&s_comma; 315: 1136-1140&s_comma; 2007.
2) Kawahara et al&s_comma; EMBO Rep.&s_comma; doi: 10.1038/sj.embor.7401011&s_comma; 2007.
3) Kawahara et al&s_comma; Nature&s_comma; 427: 801&s_comma; 2004.


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理化学研究所 脳科学総合研究センター
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